I want to auction a DC of ender pearls but they are in stacks of 16. Is that okay or do i have to auction them off in 4 dc's?
no you can auction off a double chest of them the same as how you can auction of a double chest of lava buckets, even though they don't stack to 64 it still counts as a double chest of that item.
An easy way to make sure it's legal is to just take a screenshot of what you're offering for sale. That way, people know exactly what they're bidding on and there's no confusion.